Influence of husk on grain contamination by fusarium spp. and alternaria spp. in hulled spelt (triticum spelta l.)

Karel Suchy1, Petr Konvalina1, Ivana Capouchova2, Dagmar Janovska3, Leona Leisova-Svobodova3, Zdenek Sterba1, Jan Moudry jr.1, Daniel Bucur4, Jaroslav Bernas1, Marek Kopecky1, Dang Khoa Tran1

1 University of South Bohemia in Ceske Budejovice, Faculty of Agriculture, Studentska 1668, 370 05 Ceske Budejovice, Czech Republic
2 Czech University of Life Sciences in Prague, Kam ck 129, Prague, Czech Republic
3 Crop Research Institute in Prague, Drnovska 507/73. 161 06 Praha 6 Ruzyne, Czech Republic
4 The University of Agricultural Sciences and Veterinary Medicine Iasi, Faculty of Agriculture, Aleea Mihail Sadoveanu no.3, 700490Iasi, Romania


Fusarium Head Blight is caused by several Fusarium species. Infections can result in mycotoxin contamination on cereals and associated foods. Harvested products are contaminated due to its secondary metabolites. The aim was to analyse the occurrence of spike Fusarium and Alternaria spp. in hulled Triticum spelta L. wheat species via polymerase chain reaction (PCR) method and the deoxynivalenol (DON) content analysis. Three varieties of spelt were used (Ceralio and Rubiota winter and one spring form variety from genetics resources). Grains were sown in a randomized complete block design on organic certified experimental parcels during the years of 2011 and 2013. During the vegetation period plants were artificially inoculated with Fusarium spp. The occurrence of spike Fusarium and Alternaria spp. was assessed by the PCR method -DNA extracting and determination of Fusarium species and Alternaria spp. by the DNA markers and PCR method. DON content was analysed by ROSA -DON Quantitative test. Strong infestation of grains with Fusarium spp. led to low contamination of grains with Alternaria spp. The technological operation of grain dehulling was performed and it was highly efficient there. The grain contamination by Fusarium spp. and Alternaria spp. decreased. Hulls protect grains to a certain point because of protection against Fusarium spp. and Alternaria spp. occurrence which produce harmful secondary metabolites. On the other hand the protection of grain by hulls only partly works. It is also important to pay attention to chemism of secondary metabolites in grain.


Alternaria spp.; contamination; Fusarium spp.; husk; wheat

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