Phenolic content and antioxidant activity of extracts from bifurcaria bifurcata alga, obtained by diverse extraction conditions using three different techniques (hydrothermal, ultrasounds and supercritical co2)

Ruben Agregan1, Paulo E.S. Munekata2, Daniel Franco1, Ruben Dominguez1, Javier Carballo3, Voster Muchenje4, Francisco J. Barba5, Jose M. Lorenzo1

1 Centro Tecnol gico de la Carne de Galicia, Adva. Galicia n 4, Parque Tecnol gico de Galicia, San Cibrao das Vi as, 32900 Ourense, Spain
2 Department of Food Engineering, Faculty of Animal Science and Food Engineering, University of S o Paulo, 225 Duque de Caxias Norte Ave, Jardim Elite, postal code 13.635-900, Pirassununga, S o Paulo, Brazil
3 Area de Tecnologia de los Alimentos, Facultad de Ciencias de Ourense, Universidad de Vigo, 32004 Ourense, Spain.
4 Department of Livestock and Pasture Science, University of Fort Hare, Private Bag X 1314, Alice, South Africa
5 Nutrition and Food Science Area, Preventive Medicine and Public Health, Food Science, Toxicology and Forensic Medicine Department, Universitat de Val ncia, Avda. Vicent Andr s Estell s, s/n, Burjassot, 46100 Val ncia, Spain


Extracts of Bifurcaria bifurcata seaweed were obtained by diverse conditions. Different extraction techniques, such as hydrothermal and ultrasounds, and three different solvents (water, ethanol and water/ethanol (50:50)) depending on technique were used. Moreover, supercritical CO2 (SC-CO2) with 10% of ethanol as co-solvent using different extraction times (30, 45 and 60 min) was also used as extraction technique. Extraction yield, phenolic content and antioxidant activity were measured for each extract. Hydrothermal extraction obtained better extraction yields than ultrasound extraction. Regarding the effect of solvent composition, water/ethanol (50:50) in hydrothermal treatment (HW50E50) and water/ethanol (50:50) in ultrasound treatment (UW50E50) showed the highest extraction yields. The worst extraction yields were shown by the extraction with SC-CO2. Water/ethanol (50:50) showed to be more efficient extracting phenolic compounds than water, although the highest extraction was achieved by ethanol. On the other hand, ultrasound-assisted extraction seemed to be more efficient extracting phenolic compounds than hydrothermal extraction. From the results obtained, it can be concluded that the use of ultrasound extraction technique and the use of water/ethanol as extracting solvent seemed to be the best extraction condition.


Bifurcaria bifurcata; hydrothermal; supercritical CO2; ultrasound

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