Optimizing bioremediation of hydrocarbon polluted soil by life cycle assessment (lca) approach

Giuliana D'Imporzano1,2,7, Ilaria Re1, Federica Spina3, Cristina Varese3, Edoardo Puglisi4, Giulia Spini4, Carla Gramellini5, Giacomo Zaccanti5, Fabrizio Beltrametti5 Adriana Bava6, Fabrizio Adani2,7

1 Consorzio Italbiotec, Via Fantoli 16, 20100 Milano MI Italy
2 Universit degli Studi di Milano, Via Celoria 2, 20100 Milano MI Italy
3 Universit di Torino, Viale Mattioli 25, 10125 Torino TO Italy
4 Universit Cattolica del Sacro Cuore, Via Emilia Parmense 84, 29122 Piacenza PC Italy
5 Arpae Emilia Romagna, Via Po 5, 40139 Bologna BO Italy
6 Actygea Srl, Via R. Lepetit, 34 21040 Gerenzano,VA Italy
7 Universit degli Studi di Milano, Via Celoria 2, 20100 Milano MI Italy


In the former Carbochimica site of Fidenza, a bioremediation approach was developed using the technique of biodegradation of pollutants thanks to a selected autochthonous bacterial-fungal consortium. The soil was heavily polluted up to values of total hydrocarbon equal to 1800 mg/kg. The consortium was selected from the microorganism living in the Fidenza soil, bioaugmented and finally reinoculated in the bio-pile for soil treatment. The approach is absolutely innovative, due to the presence not only of bacterial strains but also for the use of fungal strains operating in synergy with the bacteria. The first data from the trials show an effective soil remediation performance. The LCA analysis allowed to make a global assessment of the environmental impacts of the bio-pile remediation treatment scenario compared to the no-action scenario. Impacts were assessed on 18 impact categories at the midpoint level according to the ReCiPe method. For the bio-augmented bio-pile remediation, the results showed a value in the climate change category of 10 kg CO2 for each ton of remediated soil, and at the same time improvement in the categories relating to the toxicity at the local level.


bio-augmentation; bio-remediation; hydrocarbon; LCA

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