Extraction and characterization of sericin protein from bombyx mori

Inderchand Manjubala1, Yash Rupeshkumar Somaiya1, Chong Zhen Ye1, Ganesan Priya1, Uttamchand Narendrakumar2

1 Department of Biosciences, School of Bio Sciences and Technology, Vellore Institute of Technology, Vellore, India
2 Department of Manufacturing, School of Mechanical Engineering, Vellore Institute of Technology, Vellore, India


Silk sericin is a water-soluble macromolecular protein obtained from the raw cocoons of silkworm, Bombyx mori. It comprises of about 25% of the total cocoon shell weight and is categorized as a waste by-product in textile and silk industries after the extraction of silk fibers. This study emphasizes on the extraction of sericin from B. mori by a conventional salt alkaline method and characterized their physico-chemical properties, thermal stability, anti-bacterial and anti-oxidant activities. The extraction yield of the sericin protein from the cocoon was found to be 24 %. FTIR spectrum shows the presence of functional groups corresponding to the amino acids of sericin depicting its purity. TGA analysis demonstrates the degradation of sericin at 210 C with a weight loss of 57.69%. Sericin exhibited effective antibacterial activity against E. coli and S. aureus. DPPH assay revealed the antioxidant property of the sericin.


antibacterial; B. mori; extraction; protein; sericin

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